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Merck
CN

P9187

OPD

SIGMAFAST, peroxidase substrate, chromogenic, tablet

别名:

OPD过氧化物酶底物

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关于此项目

UNSPSC Code:
12352204
NACRES:
NA.83
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产品名称

SIGMAFAST OPD, tablet

form

tablet

Quality Level

solubility

water: 0.5 tablet/10 mL, clear, colorless

storage temp.

2-8°C

Application

SIGMAFAST OPD(o-苯二胺二盐酸盐)片剂用作可溶底物,在酶免疫分析(EIA)中检测过氧化物酶活性。OPD作为EIA底物是因为它有高灵敏度。 使用OPD的EIA可以在计时分析中读数或用稀释的酸性溶液终止反应,过后在读数。
SIGMAFAST OPD已用于唾液病毒样颗粒(VLP)结合测定。它还已用于开发检测大鼠血清样品中的利妥昔单抗的酶联免疫吸附测定(ELISA)板。

Features and Benefits

SIGMAFAST OPD片剂不需要另外的缓冲液或步骤来制备活性底物溶液。

Preparation Note

一片OPD片剂和一片尿素过氧化氢/缓冲液片剂,溶解在20ml水中,提供20ml即用底物。 如果按照该方法溶解片剂,得到的终浓度是0.4 mg/ml OPD, 0.4 mg/ml尿素过氧化氢和0.05 M磷酸柠檬酸盐,pH5.0。片剂是独立铝膜包装。
将每组片剂溶解在20mL去离子水中,就能生成即用型含有OPD和尿素过氧化氢缓冲溶液。

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

该底物吸湿且光敏感


signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 3



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

ELISA实验方案提供了进行间接ELISA和捕获ELISA的详细说明。了解进行夹心式ELISA和间接ELISA实验的推荐产品和技术信息。

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

商品

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.


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全球贸易项目编号

货号GTIN
P9187-50SET04061835509331
P9187-5SET04061835558889