added to the 96-well plate and the absorbance read
affinity chromatography. Monoclonal Anti-IL-6 at 540 nm.
Receptor is provided as a liquid in phosphate buffered
saline to which no preservatives are added
, and scored for cytopathic effect by
measurement of optical densities in a microplate reader
at 540 nm.
The Neutralization Dose50 (ND50) for Anti-IFN-γ is
0.08-0.2 µg/mL in the presence of 2.5 ng
Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine hydrochloride
Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine hydrochloride
Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine hydrochloride
Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine hydrochloride
Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine hydrochloride
Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine hydrochloride
Neutral Red Neutral Red (3-Amino-7- Viable cells as measured by Neutral red is taken up by 540 nm 11,12
dimethylamino-2-methyl- uptake of the dye viable cells and stored in the 13,14
phenazine hydrochloride
,
540, 1990.
5. Meyskens, F.L., Jr. and Fuller, B.B., Cancer Res.
40, 2194, 1980.
6. Verma, A.K. et al., Cancer Res. 38, 793, 1978.
7. Jansz, A. et al., Int. Arch.
Neurochem. 54, 540, 1990.
10. Meyskens, F.L., Jr. and Fuller, B.B., Cancer Res. 40, 2194, 1980.
11. Verma, A.K. et al., Cancer Res. 38, 793, 1978.
12. Jansz, A. et al., Int. Arch. Allergy 31, 69, 1967.
13
582-588.
L Schiefer, S. & Beutler, H-O. (1985) „MEA“ 8, 87-104.
P Brolin, S.E. (1983) „MEA“ 3, 540-545.
F Ziegenhorn, J. et al. (1976) Clin. Chem. 22, 151-160.
Keppler, D. (1985) „MEA“ 7, 322-332
References 1 Loh,Y. et al. (1989) Science 243, 217.
2 Ohara et al. (1989) Proc. Natl. Acad. Sci USA 86, 5673.
3 Frohmann, M. (1994) PCR Methods and Applications 4, 540–558.
4 Saiki, R. (1990) PCR Protocols: A Guide
mix to bring all salts into
solution. Dilute 60 mL of 10X Wash Buffer with 540 mL deionized water. Store
unused portions at 2-8°C for up to one month.
D. Preparation of Serum Matrix
This