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Enzyme activity assays
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facet applications:Enzyme activity assays
facet content type:Protocol
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食品酶法分析
用于食品分析的酶法具有高度特异性，与其他方法相比，特别是从样品制备的角度来看，可以节省大量时间和成本。我们提供各种方便的试剂盒和试剂，用于快速可靠的酶食品分析。
以酪蛋白为底物酶法测定蛋白酶
建立以酪蛋白为底物的蛋白酶的标准酶法测定方法。
GST Detection Module with CDNB Enzymatic Assay
This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from GE Healthcare.
Enzymatic Assay of Glucose Oxidase
Enzymatic Assay of Glucose Oxidase
变溶菌素的酶促检测
该操作步骤可用于变溶菌素产物。
Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)
To standardize a procedure for the enzymatic assay of Diamine Oxidase.
6-磷酸葡萄糖脱氢酶（EC 1.1.1.49）的酶学测定
6-磷酸葡萄糖脱氢酶（EC 1.1.1.49）的酶学测定
过氧化氢酶的酶学测定（EC 1.11.1.6）
本程序适用于所有过氧化氢酶产品。
醇脱氢酶（EC 1.1.1.1）的酶学测定
该程序可用于醇脱氢酶产物。
Enzymatic Assay of Ficin (EC 3.4.22.3)
This procedure may be used for all Ficin products.
脂肪酶活性测定实验方案
脂肪酶活性测定实验方案
酸性磷酸酶的酶促测定（EC 3.1.3.2）
酸性磷酸酶的酶促测定（EC 3.1.3.2）
抑肽酶酶活测定
目标：建立抑肽酶酶活测定的标准化操作步骤。
Assay Procedure for Invertase
The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.
通过p24（病毒外壳蛋白）ELISA法测定慢病毒滴度
通过p24 ELISA法计算慢病毒滴度。详细的实验方案如下。
Enzymatic Assay of Proteinase K (EC 3.4.21.64)
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol
Enzymatic Assay of Nuclease S1
Enzymatic Assay of Nuclease S1
MTT Assay Protocol for Cell Viability and Proliferation
MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.
Substrates Procedures
Our General Protease Assay Procedures and Substrates overview.
胰蛋白酶（EC 3.4.21.4）的酶学测定方案
本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯（BAEE）作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定（A253，光程= 1cm）方案：
Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)
This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.
Enzymatic Assay of Choloylglycine Hydrolase
To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
5'-核苷酸酶的酶促检测
目标：建立5'-核苷酸酶酶促测定的标准化操作流程。
Enzymatic Assay of Sulfatase (EC 3.1.6.1.)
Enzymatic Assay of Sulfatase (EC 3.1.6.1.)
溶菌酶的酶学测定（EC 3.2.1.17）
该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。
Enzymatic Assay of Fumarase
Enzymatic Assay of Fumarase
转化酶的酶学测定
转化酶的酶学测定
Enzymatic Assay of Ferredoxin NADP Reductase (EC 1.18.12)
Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).
改良Boehenger法酶促检测α-葡萄糖苷酶(EC 3.2.1.20)
改良Boehenger法酶促检测α-葡萄糖苷酶(EC 3.2.1.20)
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