Nucleic acid labeling and detection

应用筛选条件

facet applications:Nucleic acid labeling and detection

facet content type:Protocol

显示 1-26 共 26 条结果

存在有多种可与BM Purple（或通常的NBT/BCIP）组合的复染剂，包括FastGreen FCF和Nuclear Fast Red。

There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), for instance, FastGreen FCF or Nuclear Fast Red.

Random Primed DNA Labeling Kit Protocol & Troubleshooting

PTK/PTP Expression Profiling

NBT/BCIP Ready-to-Use Tablets Protocol & Troubleshooting

Biotin-16-dUTP Protocol & Troubleshooting

Protocol for Biotin-16-dUTP Protocol & Troubleshooting

DIG RNA Labeling Mix Protocol & Troubleshooting

Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).

NBT/BCIP Stock Solution Protocol & Troubleshooting

NBT/BCIP signals should not be mounted with xylene containing mounting media (e.g., DPX), because these could lead to crystal formations of the color precipitates. Classical counterstains, such as eosin, however, require xylene containing mounting media.

DIG Easy Hyb™ Granules Protocol & Troubleshooting

Protocol for DIG Easy Hyb™ Granules Protocol & Troubleshooting

DIG Northern Starter Kit Protocol & Troubleshooting

In general, it is possible to use DNA probes for northern blots (PCR or random-primed DNA probes). It is however important to keep in mind that DNA probes are best detecting abundant messages, such as housekeeping genes.

DIG RNA Labeling Kit (SP6/T7) Protocol & Troubleshooting

Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).

DIG Easy Hyb™ Granules Protocol & Troubleshooting

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

DIG RNA Labeling Kit (SP6/T7) Protocol & Troubleshooting

Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).

NBT Protocol

DIG RNA Labeling Mix Protocol & Troubleshooting

NBT/BCIP Stock Solution Protocol & Troubleshooting

Random Primed DNA Labeling Kit Protocol & Troubleshooting

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

Dextran Sulfate

Dextran sulfates are supplied as the sodium salt forms, making them soluble and stable in water. Dextran sulfate contains approximately 17% sulfur which is equivalent to approximately 2.3 sulfate groups per glucosyl residue.

Oligonucleotide Melting Temperature

Calculate the melting temperature (Tm) of oligonucleotides using theoretical method like the nearest neighbor or the basic method. The DNA Tm is the temperature at which 50% of the oligonucleotide is duplexed with its perfect complement and 50% is free

Biotin-16-dUTP Protocol & Troubleshooting

PTK/PTP Expression Profiling

ABI3900 Synthesizer Columns with CUTAG CPG Frits

Next generation CPG Frit support for reliable, cost-effective oligo synthesis from Proligo®, for ABI 3900 and MerMade synthesizers

NBT/BCIP Ready-to-Use Tablets Protocol & Troubleshooting

DIG Northern Starter Kit Protocol & Troubleshooting