CN
Cloning and Expression
应用筛选条件
facet applications:Cloning and Expression
facet content type:Technical Article
显示 1-30 共 44 条结果
Troubleshooting in pGEX Expression Vectors
This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.
Simplicon™ In Vivo Protein Expression System
Simplicon: A non-genome integrating, tunable and sustained protein expression system in human cells, based on a single, polycistronic, and self-replicating synthetic RNA.
Plasmid Product Nomenclature
The SnapFast system is a versatile plasmid cloning platform that provides a range of functional DNA sequences in an easy to clone format. Hundreds of pre-designed DNA sections that can be easily incorporated into, or transferred between, our range of
Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
蓝白斑筛选和菌落筛选实验方案
了解并寻找分子生物学研究所用的蓝白斑筛选技术实验方案，以鉴定重组细菌克隆并进行进一步分析。
限制性内切核酸酶 — 分子剪
术语“限制酶”源自Werner Arber和Matthew Meselson实验室对肠杆菌噬菌体λ（λ噬菌体）的研究。
How Transfection Works
This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.
FLAG®标记细菌和哺乳动物表达载体
FLAG®和3xFLAG®表达载体和产品用于细菌和哺乳动物表达载体以及蛋白质检测和纯化。
SIG10 Chemically Competent Cells
For best results, ligation reactions must be heat inactivated at 70º C for 15 minutes before transformation. Alternately, the reactions may be purified.
Cloning & Expression Vectors
Expression Vectors for Bacterial, Mammalian and Insect Cell Systems. Featured are a variety of tags, promoters and elements for secretion, transient, stable and bicistronic expression.
SnapFast™ Restriction Site Functions
Learn more about relevant restriction site functions in the SnapFast™ plasmid system. All DNA sections are pre-screened, and where possible modified, to remove any of the restriction sites found within the core SnapFast plasmids to maintain their flexibility.
EnPresso FAQs
Frequently asked question about EnPresso.
Reverse transfection of plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)
A helpful chart for selection of correct nucleic acid modifying enzyme.
T7 Promoter System
Bacterial Expression Vectors: T7 Promoter System. T7 Vectors for Highest Expression Levels in Bacteria.
Restriction Enzyme Cloning Glossary
Restriction Enzyme Cloning Glossary
Synthesis and Biomedical Applications of Polyamino Acids
Polyamino acids are able to adopt ordered conformations, such as α-helices and β-sheets, through cooperative hydrogen bonding. These conformations impart polyamino acids with various unique properties and functions in biological environments.
Troubleshooting in pGEX Expression Vectors
This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector using GE Healthcare products.
Restriction Enzyme Cloning Glossary
Restriction Enzyme Cloning Glossary
Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
T7 Promoter System
Bacterial Expression Vectors: T7 Promoter System. T7 Vectors for Highest Expression Levels in Bacteria.
Troubleshooting for Molecular Cloning
Molecular cloning is the process of inserting the gene-of-interest (GOI) into a plasmid vector and this vector is then inserted into a cell that expresses the protein encoded by the GOI. Once protein is expressed in the cell, the protein
Ligation of Insert to pGEX DNA
This page describes ligation of insert to pGEX DNA using GE Healthcare products.
Restriction Endonucleases - The Molecular Scissors
The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.
Introduction to Cell Transfection
Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions
Competent Cell Selection & E. coli Markers Guide
Select competent cells by application, transformation efficiency, and genotype. We also present a list of E. coli markers for reference.
Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)
A helpful chart for selection of correct nucleic acid modifying enzyme.
Synthesis and Biomedical Applications of Polyamino Acids
Synthesis and Biomedical Applications of Polyamino Acids
Transforming E.coli with Engineered Plasmid
Making Competent Cells; Making Agar Plates; Bacterial Transformation; Picking Colonies; Growing Bacteria in Liquid Culture; Freezing Bacteria.
Reverse transfection of plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
1/2