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关键词:'P4860'
显示 31-52 共 52 条结果 关于 "P4860" 范围 论文
Subhash Pokharel et al.
Nucleic acids research, 40(16), 7821-7830 (2012-06-12)
Dna2 nuclease/helicase is a multitasking protein involved in DNA replication and recombinational repair, and it is important for preservation of genomic stability. Yeast Dna2 protein contains a conserved putative Fe-S (iron-sulfur) cluster signature motif spanning the nuclease active site. We
Poonam R Bajaj et al.
Journal of microencapsulation, 34(2), 218-230 (2017-04-11)
Unhydrolysed pea protein (UN) forms very viscous emulsions when used at higher concentrations. To overcome this, UN was hydrolysed using enzymes alcalase, flavourzyme, neutrase, alcalase-flavourzyme, and neutrase-flavourzyme at 50 °C for 0 min, 30 min, 60 min, and 120 min to form hydrolysed proteins A
Teodolinda Petrillo et al.
Biochemistry, 51(31), 6164-6170 (2012-07-05)
Two new inhibitors in which the terminal α-carboxyl groups of Z-Ala-Ala-Phe-COOH and Z-Ala-Pro-Phe-COOH have been replaced with a proton to give Z-Ala-Ala-Phe-H and Z-Ala-Pro-Phe-H, respectively, have been synthesized. Using these inhibitors, we estimate that for α-chymotrypsin and subtilisin Carlsberg the
Renxian Wang et al.
Phytochemistry, 95, 118-126 (2013-08-06)
Senescence-related proteases play important roles in leaf senescence by regulating protein degradation and nutrient recycling. A 98.9kDa senescence-related protease EP3 in wheat leaves was purified by ammonium sulfate precipitation, Q-Sepharose fast flow anion exchange chromatography and gel slicing after gel
Nadeem Javid et al.
The journal of physical chemistry letters, 2(12), 1395-1399 (2011-06-16)
The structural characterization of subtilisin mesoscale clusters, which were previously shown to induce supramolecular order in biocatalytic self-assembly of Fmoc-dipeptides, was carried out by synchrotron small-angle X-ray, dynamic, and static light scattering measurements. Subtilisin molecules self-assemble to form supramolecular structures
Youské Shimizu et al.
The FEBS journal, 280(7), 1681-1692 (2013-02-13)
Members of the kinesin-13 sub-family, including KIF2C, depolymerize microtubules. The positive charge-rich 'neck' region extending from the N-terminus of the catalytic head is considered to be important in the depolymerization activity. Chemically synthesized peptides, covering the basic region (A182-E200), induced
Kusum Solanki et al.
Bioresource technology, 115, 147-151 (2012-01-18)
A first study of the comparison of structures of enzymes (by FT-IR and CD) in different high activity (in low water media) preparations is reported. Using chymotrypsin and subtilisin as models, we have studied various factors that distinguish enzyme precipitated
P Nedkov et al.
Biological chemistry Hoppe-Seyler, 366(4), 421-430 (1985-04-01)
The complete amino-acid sequence of subtilisin DY, an extracellular alkaline proteinase produced by Bacillus subtilis strain DY was determined. This included automated sequence analysis of the whole molecule and its large fragments such as tryptic peptides obtained from the inactivated
Bo Wang et al.
Food chemistry, 218, 1-8 (2016-10-11)
The transepithelial transport routes of casein-derived peptides with different molecular weights (MWs) were investigated using a Caco-2 cell monolayer. The peptidase hydrolysis during transport was also studied. The results indicate that the paracellular route was the main pathway for F1
Derya Y Koseoglu-Imer et al.
Colloids and surfaces. B, Biointerfaces, 92, 334-339 (2012-01-06)
In this study, the surface of cellulose acetate (CA) ultrafiltration membrane was activated with serine protease (Savinase) enzyme to reduce protein fouling. Enzyme molecules were covalently immobilized with glutaraldehyde (cross-linking agent) onto the surface of CA membranes. The membrane activation
Bastien Mangeat et al.
The Journal of biological chemistry, 287(26), 22015-22029 (2012-04-12)
Influenza virus infections lead to a burst of type I interferon (IFN) in the human respiratory tract, which most probably accounts for a rapid control of the virus. Although in mice, IFN-induced Mx1 factor mediates a major part of this
Andrew R Hirst et al.
Nature chemistry, 2(12), 1089-1094 (2010-11-26)
Supramolecular gels, which demonstrate tunable functionalities, have attracted much interest in a range of areas, including healthcare, environmental protection and energy-related technologies. Preparing these materials in a reliable manner is challenging, with an increased level of kinetic defects observed at
Savvas Saouros et al.
The Journal of biological chemistry, 287(43), 36029-36040 (2012-08-17)
Toxoplasma gondii is the model parasite of the phylum Apicomplexa, which contains obligate intracellular parasites of medical and veterinary importance. Apicomplexans invade host cells by a multistep process involving the secretion of adhesive microneme protein (MIC) complexes. The subtilisin protease
Daixi Liu et al.
Gene, 514(2), 99-104 (2012-12-04)
A 255-bp cDNA encoding an 84-amino acid residue (aa) precursor protein containing 8 half-cysteines was cloned from the skin of the frog, Ceratophrys calcarata. By sequence comparison and signal peptide prediction, the precursor was predicted to release a 63-aa mature
Lukas Miksch et al.
Polymers, 13(6) (2021-04-04)
Bio-based polymers have been suggested as one possible opportunity to counteract the progressive accumulation of microplastics in the environments. The gradual substitution of conventional plastics by bio-based polymers bears a variety of novel materials. The application of bioplastics is determined
Younes Ghasemi et al.
Molecular biotechnology, 52(1), 1-7 (2011-11-10)
Several investigations are being pursued to enhance the efficacy and specificity of fibrinolytic therapy. In this regard, microbial fibrinolytic enzymes attracted much more medical interests during these decades. Subtilisin, a member of subtilases (the superfamily of subtilisin-like serine proteases) and
Shiyi Lu et al.
Food & function, 12(3), 1135-1146 (2021-01-13)
Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.
Srinidhi Jayaraman et al.
The Journal of pharmacology and experimental therapeutics, 379(2), 191-202 (2021-08-15)
Neurolysin (Nln) is a recently recognized endogenous mechanism functioning to preserve the brain from ischemic injury. To further understand the pathophysiological function of this peptidase in stroke and other neurologic disorders, the present study was designed to identify small molecule
Esra Uçkun Kiran et al.
Enzyme and microbial technology, 50(6-7), 337-342 (2012-04-17)
Rapeseed meal, a major byproduct of biodiesel production, has been used as a low-cost raw material for the production of a generic microbial feedstock through a consolidated bioconversion process. Various strategies were tested for the production of a novel fermentation
Priyanka Singh Rao et al.
Journal of food science and technology, 57(12), 4562-4575 (2020-10-23)
This paper shows the potential of dual enzyme approach on antioxidant activity of casein hydrolysates. Casein was hydrolysed using the proteolytic enzymes alcalase, flavourzyme in isolation and in sequential order. Casein hydrolysates were evaluated for the degree of hydrolysis, antioxidant
Thibault Leger et al.
Free radical biology & medicine, 153, 71-79 (2020-04-25)
Evaluating the activity of cardiac mitochondria is probably the best way to estimate early cellular damage in chronic pathology. Early diagnosis allows rapid therapeutic intervention thus increasing patient survival rate in a number of diseases. However, data on human cardiac
Chrystelle Derache et al.
The FEBS journal, 279(24), 4466-4478 (2012-10-19)
Greglin is an 83-residue serine protease inhibitor purified from the ovaries of the locust Schistocerca gregaria. Greglin is a strong inhibitor of subtilisin and human neutrophil elastase, acting at sub-nanomolar and nanomolar concentrations, respectively; it also inhibits neutrophil cathepsin G, α-chymotrypsin and
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