Merck
CN
  • HU protein is involved in intracellular growth and full virulence of Francisella tularensis.

HU protein is involved in intracellular growth and full virulence of Francisella tularensis.

Virulence (2018-02-24)
Pavla Stojkova, Petra Spidlova, Juraj Lenco, Helena Rehulkova, Lucie Kratka, Jiri Stulik
摘要

The nucleoid-associated HU proteins are small abundant DNA-binding proteins in bacterial cell which play an important role in the initiation of DNA replication, cell division, SOS response, control of gene expression and recombination. HU proteins bind to double stranded DNA non-specifically, but they exhibit high affinity to abnormal DNA structures as four-way junctions, gaps or nicks, which are generated during DNA damage. In many pathogens HU proteins regulate expression of genes involved in metabolism and virulence. Here, we show that the Francisella tularensis subsp. holarctica gene locus FTS_0886 codes for functional HU protein which is essential for full Francisella virulence and its resistance to oxidative stress. Further, our results demonstrate that the recombinant FtHU protein binds to double stranded DNA and protects it against free hydroxyl radicals generated via Fenton's reaction. Eventually, using an iTRAQ approach we identified proteins levels of which are affected by the deletion of hupB, among them for example Francisella pathogenicity island (FPI) proteins. The pleiotropic role of HU protein classifies it as a potential target for the development of therapeutics against tularemia.

材料
货号
品牌
产品描述

Millipore
Benzonase®核酸酶, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
Sigma-Aldrich
StableCell DMEM - 高葡萄糖, With 4500 mg/L glucose, stable glutamine, and sodium bicarbonate, without sodium pyruvate., liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
蛋白标准品, Micro Standard, liquid
Millipore
Amicon® Pro Affinity Concentration Kit Protein G with 3kDa Amicon® Ultra-0.5 Device, Amicon Pro Affinity Concentration Kit Protein G - A Centrifugal Tool for purifying & concentrating Antibodies.