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HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX (Ion Exchange)

HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX (Ion Exchange) application for HPLC

材料

标准

产品编号
说明
价格

C4011

细胞色素 C 来源于鸽子胸肌

≥95% based on Mol. Wt. 12,173 basis

C2037

线粒体蛋白 来源于牛心脏

≥95% based on Mol. Wt. 12,327 basis

C3131

线粒体蛋白 来源于牛心脏

≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

C7150

细胞色素 C 来源于马心脏

suitable for GFC marker, BioReagent

C7752

细胞色素 C 来源于马心脏

≥95% based on Mol. Wt. 12,384 basis

C2506

细胞色素 C 来源于马心脏

≥95% (SDS-PAGE)

C2436

细胞色素 C 来源于酿酒酵母

≥85% based on Mol. Wt. 12,588 basis

C3483

线粒体蛋白 来源于人类心脏

≥95% (SDS-PAGE), lyophilized powder

30398

细胞色素 C 来源于牛心脏

≥95% (GE)

C2867

细胞色素 C 来源于马心脏

BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture

CONDITIONS

column

Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U)

mobile phase

[A] 20 mM Bis-Tris HCl, pH 7.0; [B] 20 mM Bis-Tris HCl, 0.5M NaCl, pH 7.0; 24 to 69% B in 20 min. (linear)

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 280 nm

injection

10 μg each variant

说明

分析说明

This Figure shows the separation of three cytochrome c variants on Discovery BIO PolyMA-SCX. Note the difference in elution order compared to the reversed-phase separation on Discovery BIO Wide Pore C18 shown in product G001581.

法律信息

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS