The HighPrep™ PCR Purification System uses magnetic beads-based chemistry without centrifugation or filtration in a simple 3-step procedure that includes a bind, wash, and elution step. After HighPrep™ PCR is added to the PCR reaction sample, the protocol utilizes a magnet plate (magnet stand) for processing the PCR reaction sample. During the process, contaminants and salts are washed off and pure DNA is eluted and ready to be used in subsequent applications.
HighPrep™ PCR Clean Up Kit
Equipment and reagents to be supplied by user:
Reaction Plate:
Product Specifications
Number of reactions is based on typical 10µL PCR reaction volume. Volume of HighPrep™ PCR reagent per reaction = 1.8 x (PCR Reaction Volume)
Be sure to bring the HighPrep™ PCR reagent to room temperature for at least 30 min before use.
Formula used to calculate the volume of HighPrep™ PCR reagent needed for PCR reaction: HighPrep™ PCR reagent volume per reaction = 1.8 X PCR reaction volume.
IMPORTANT: Do not disturb the attracted beads while aspirating the supernatant.
IMPORTANT: It is critical to completely remove all traces of alcohol but take caution in not over drying the beads as this will reduce the yield.
Formula used to calculate the volume of HighPrep™ PCR reagent needed for PCR reaction: HighPrep™ reagent volume per reaction = 1.8 X PCR reaction volume.
IMPORTANT: Do not disturb the attracted beads while aspirating the supernatant.
IMPORTANT: It is critical to completely remove all traces of alcohol but take caution in not over drying the beads as this will reduce the yield.
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