D0547
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
DME/F-12, 1:1 mixture
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About This Item
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:
- to culture cerebral cortices from mice embryos
- as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
- to culture human corneal epithelial cell lines
Preparation Note
Formulated to contain 12.0 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate
Other Notes
Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate
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Protocols
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Articles
Nutrient Mixture F-12 Ham Formulation