Product Name
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM
shipped in
ambient
storage temp.
2-8°C
General description
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:
- Hep-2 cells
- mouse fibroblast L‐cells
- L‐ Wnt family member 3A (Wnt3a) cells
- primary buffalo embryonic fibroblast (BEF)
Preparation Note
Formulated to contain 15.6 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.
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Storage Class
11 - Combustible Solids
wgk
WGK 1
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Protocols
粉末培养基及盐混合物极具吸湿性,应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Articles
Nutrient Mixture F-12 Ham Formulation