D7295
Deoxynucleotide Mix, 10 mM
Molecular Biology Reagent
Synonym(s):
Deoxynucleotide Mix, 10 mM, 10mM dNTP mix, dNTP mix, dNTPs
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About This Item
NACRES:
NA.52
UNSPSC Code:
41106305
Assay:
≥99%
Biological source:
synthetic (organic)
Form:
liquid
Solubility:
H2O: soluble at 20 °C
Storage temp.:
−20°C
biological source
synthetic (organic)
Quality Level
assay
≥99%
form
liquid
concentration
10 mM
color
colorless
solubility
H2O: soluble at 20 °C
application(s)
agriculture
foreign activity
DNase, RNase, none detected
shipped in
dry ice
storage temp.
−20°C
General description
Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.
Application
dNTP Mix has been used:
- in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,
- in reverse transcription of total RNA to cDNA.
- as a component of the DNA amplification mixture for polymerase chain reaction (PCR)
- routine and long PCR
- manual and automated DNA sequencing
- cDNA synthesis and labeling reactions
Features and Benefits
- Purity of each dNTP: Minimum 99%
- Conveniently formulated; 1 μL is used per 50 μL PCR
- Equimolar amounts of each dNTP means less pipetting
- Minimize risk of contamination in PCR
- UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.
了解标准 PCR 方案步骤，查看试剂清单或循环参数。本方法使用标准 Taq DNA 聚合酶对 DNA 进行常规 PCR 扩增。