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D8900

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Name: Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
Brand: SIGMA

With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

DMEM Hams F12, DME/F-12, 1:1 mixture

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.71

MDL number:

MFCD00217417

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Properties

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO₃: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

Description

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:

as a component of the culture medium for endometrial explants in vitro culture
to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein

Preparation Note

Formulated to contain 15.6 grams of powder per liter of medium.

Supplement with 1.2 g/L sodium bicarbonate.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

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Protocols

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Articles

Nutrient Mixture F-12 Ham Formulation

Water for Mammalian Cell Culture

Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.