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TP0300

Total Protein Kit, Micro Lowry, Peterson′s Modification

Name: Total Protein Kit, Micro Lowry, Peterson′s Modification
Brand: SIGMA

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

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Properties

usage

sufficient for ~200 assays

Quality Level

200

Description

General description

This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.

Application

Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.

Biochem/physiol Actions

An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.

Features and Benefits

Complete kit for protein assays
Most widely cited protein assay
Less protein to protein variation than other assays
Contains reagents that can remove interfering substances

pictograms

GHS05,GHS07,GHS09

signalword

Danger

hcodes

H290,H302,H314,H335,H410

pcodes

P234 - P273 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 3

Regulatory Information

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Protocols

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.