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About This Item
material
polycarbonate membrane, polystyrene
description
sterility
γ-irradiated, sterile
shelf life
3 yr
feature
lid, automation compatible
manufacturer/tradename
Millicell®
parameter
50 °C max. temp.
technique(s)
cell attachment: suitable, cell culture | mammalian: suitable, cell differentiation: suitable, cell migration: suitable, drug transporter assay: suitable, permeability assay: suitable
L × W × H
127.76 mm × 85.48 mm × 20.32, with lid
filter diam.
9.5 mm
filtration area
0.7 cm2
plate size
24 wells
working volume
400 μL
pore size
3 μm
binding type
Tissue Culture (TC)-treated surface
shipped in
ambient
storage temp.
10-30°C
General description
The Millicell device is available with a 0.4 µm PCF and a variety of PET membrane pore sizes. The plate is designed for maximum user convenience and incorporates features such as apical assist for easier pipetting and basolateral access. To reduce the risk of monolayer contamination, the membrane plates have feet to elevate the plate above the work surface when disassembled from the feeder tray. Tear drop-shaped receiver wells eliminate air bubbles as plates are assembled.
Millicell plate are optimized to grow and sustain high integrity cell monolayers. Cells grown on Millicell plate membranes grow better than on plastic because the cells are nourished from both the apical and basolateral sides. Cell growth and function more closely mimic in vivo. Millicell plates are designed for analysis as well as cell growth and can be used manually or with automated cell seeding, feeding and washing systems
With this complete system, you can grow, feed, and analyze cells in one membrane-bottom plate. The plate can be used with either a single-well or a multi-well feeding tray. At the time of transport analysis, the plate is simply transferred to a multi-well transport tray for analysis.
This streamlined design enhances functionality with:
- Seed-and-feed systems
- Automated liquid handling systems and span pipettes
- Basolateral and apical access to cells
- Transepithelial electrical resistance (TEER) measurement systems
Other Notes
5-pack excludes single-well trays product PSSW010R5.
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Protocols
This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| PSST010R1 | 04053252505416 |
| PSST010R5 | 04053252317682 |