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Merck
CN

A5754

3-Amino-9-ethylcarbazole

≥95% (HPLC), powder

Synonym(s):

9-Ethylcarbazol-3-amine, AEC

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About This Item

Empirical Formula (Hill Notation):
C14H14N2
CAS Number:
Molecular Weight:
210.27
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
174969
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Product Name

3-Amino-9-ethylcarbazole, ≥95% (HPLC), powder

Quality Level

assay

≥95% (HPLC)

form

powder

impurities

≤3% solvent

solubility

ethanol: acetic acid (2:1): 50 mg/mL, clear to hazy

storage temp.

2-8°C

SMILES string

CCn1c2ccccc2c3cc(N)ccc13

InChI

1S/C14H14N2/c1-2-16-13-6-4-3-5-11(13)12-9-10(15)7-8-14(12)16/h3-9H,2,15H2,1H3

InChI key

OXEUETBFKVCRNP-UHFFFAOYSA-N

General description

3-Amino-9-ethylcarbazole serves as chromogen in immunocytochemistry and produces a red color.
Substrate for peroxidase.

Application

3-Amino-9-ethylcarbazole has been used to visualize the bound primary antibody by using a streptavidin method. It has also been used in visualizing tissue sections from tumour samples.

Biochem/physiol Actions

3-Amino-9-ethylcarbazole is a peroxidase substrate suitable for use in immunoblotting and immunohistochemical staining procedures. This substrate produces an insoluble end product that is red in color and can be observed visually.

Analysis Note

Actual content given on label


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pictograms

Skull and crossbonesHealth hazard

signalword

Danger

hcodes

Hazard Classifications

Acute Tox. 3 Oral - Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges



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Articles

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.