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E2636

ExtrAvidin® –Alkaline Phosphatase

buffered aqueous solution

Synonym(s):

Alkaline Phosphatase Reagent, ExtrAvidin Alkaline Phosphatase

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00163600
Form:
buffered aqueous solution
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conjugate

alkaline phosphatase conjugate

Quality Level

200

form

buffered aqueous solution

technique(s)

dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000

shipped in

ambient

storage temp.

2-8°C

General description

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques.

Application

ExtrAvidin® -Alkaline Phosphatase has been used in:
  • Dot blot
  • Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
  • Indirect ELISA
  • Enzyme linked immunosorbent assay (ELISA)
  • Enzyme-linked immunosorbent spot (ELISPOT) analysis
  • Competitive ELISA

Biochem/physiol Actions

Alkaline Phosphatase catalyzes the hydrolysis of esters to yield phosphoric acid. It is also facilitate the transport of substances across membranes.

Physical form

Solution in 0.05 M Tris-HCl buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Affinity purified protein

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
低风险生物材料

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Protocols

免疫组织化学操作步骤

本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Articles

ELISpot Assay: Functional Cellular Immunology

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.