LB Broth with agar (Lennox)

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

LB Broth with agar (Lennox) has been used for:

• the transformation of competent E. coli cells
• to cultivate Ecc-15 bacteria
• embedding scaffold containing tissue derived from induced pluripotent stem cells (iPSCs)
• transmission electron microscopy (TEM) and immunohistochemistry (IHC)

It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

Lennox LB powder with agar provides:

• A budget-friendly alternative to pre-poured plates
• Easy scale-up using larger package sizes
• Standard formulation

1. Suspend 35 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving.

Zeocin is a registered trademark of Cayla Sarl