N3910
Neutralization Solution B
Synonym(s):
DNA neutralization solution, neutralization buffer DNA extraction
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161703
grade
Molecular Biology
Quality Level
form
liquid
feature
Direct PCR
technique(s)
DNA amplification: suitable, DNA extraction: suitable, PCR: suitable
input
crude DNA
storage temp.
−20°C
General description
This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.
Application
Neutralization Solution B has been used in the modified Sigma Extract-N-Amp™ extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp™ PCR Reaction Mix.
Legal Information
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
Regulatory Information
低风险生物材料
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Protocols
SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.