P1415
Prehybridization Solution
for Northern and Southern blotting, liquid (2x concentrate)
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
General description
Sigma′s Prehybridization Solution is a 2x ready-to-use buffer for Northern and Southern blot protocols.
Application
Suitable for use as a pre-hybridization buffer in Northern and Southern blot protocols.
Preparation Note
Sigma′s 2X Prehybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
- 200 μg/mL DNA
- 2mg Bovine Serum Albumin (BSA)
- 2mg Ficoll
- 2mg Polyvinylpyrrolidone (PVP)
- 0.15 M sodium citrate
- 1.5M NaCl
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
低风险生物材料
常规特殊物品
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Protocols
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.