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P1598

PCR 20 bp Low Ladder

for electrophoresis of PCR fragments

Synonym(s):

20 bp Low Ladder, Marker for DNA gel electrophoresis

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About This Item

NACRES:
NA.25
UNSPSC Code:
41141827
Biological source:
fermentation/recombinant (E. coli strain DH5A)
Form:
liquid
Solubility:
water: soluble at 20 °C
Storage temp.:
2-8°C
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biological source

fermentation/recombinant (E. coli strain DH5A)

Quality Level

100

grade

Molecular Biology

form

liquid

usage

80 uses

solubility

water: soluble at 20 °C

suitability

suitable for electrophoresis (DNA)

storage temp.

2-8°C

General description

Sigma′s PCR 20 bp Low Ladder contains 50 dsDNA fragments from 50 – 1,000 bases, evenly spaced in 20 bp repeats. Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

PCR 20 bp Low Ladder has been used as a marker for DNA gel electrophoresis.

Other Notes

Contains 50 bands, 20–1,000 bp in exact 20 bp repeats.
For optimal resolution, the recommended agarose gel concentration is 2.5%.
Sigma′s PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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