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PP0201

Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit

sufficient for 20 samples

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About This Item

NACRES:
NA.26
UNSPSC Code:
12352202
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Quality Level

200

storage temp.

2-8°C

General description

Contains sufficient reagents for a minimum of 20 reactions when the sample size is between one to two mg of a typical glycoprotein.

Application

Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit has been used in the deglycosylation of Schizophyllum commune lectin, Arabidopsis mannanase, human umbilical vein endothelial cells (HUVEC) protein lysate and organic solute transporter protein, Ostβ.
GlycoProfile II Enzymatic In-Solution N-Deglycosylation Kit was used to study the effects of glycosylation of the N-acetyl-D-galactosamine (GalNAc)-specific lectin found in Schizophyllum commune.
GlycoProfile™ II has been optimized to provide a convenient and reproducible method to remove N-linked glycans from glycoproteins and is compatible with subsequent MALDI-TOF mass spectrometric analysis without interference from any of the reaction components.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • 10× Reaction Buffer 1 mL/vial

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • P7367
    PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE) 50 U
    SDS
  • R7884
    Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE) .5 mg
    SDS
  • O9882
    Octyl β-D-glucopyranoside, BioXtra, ≥98% (GC) 100 mg
    SDS
  • M3148
    2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) .9 mL
    SDS

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

target_organs

Liver,Heart

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

Regulatory Information

危险化学品
低风险生物材料
常规特殊物品

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Protocols

Mass Spectrometry of Glycans

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

Articles

Deglycosylation Kits

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.