S5897
Sephadex® G-50
BioReagent, DNA grade, Molecular Biology, fine
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About This Item
grade
Molecular Biology
Quality Level
product line
BioReagent
form
beads
technique(s)
DNA purification: suitable
matrix
cross-linked dextran
swelling
1 g swells to 9-11 mL
bead diameter
20-80 μm
pore size
exclusion limit (20 bp dsDNA (rA)20)
pH
2-10
foreign activity
DNase, RNase, none detected
storage temp.
room temp
SMILES string
O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O
InChI
1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
InChI key
FZWBNHMXJMCXLU-UHFFFAOYSA-N
General description
Sephadex® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.
S5897-100G′s updated product number is GE17-0573-02
S5897-100G′s updated product number is GE17-0573-02
Application
Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.
Features and Benefits
- Excellent recovery
- Minimal sample dilution
- Quick and easy to use
Legal Information
Sephadex is a registered trademark of Cytiva
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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