S6022
Sephadex® G-50
BioReagent, DNA grade, Molecular Biology, medium
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About This Item
grade
Molecular Biology
Quality Level
product line
BioReagent
form
beads
swelling
1 g swells to 9-11 mL
bead diameter
50-150 μm
pore size
exclusion limit (20 bp dsDNA; (rA)20)
pH
2-10
foreign activity
DNase, RNase, none detected
storage temp.
room temp
SMILES string
O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O
InChI
1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
InChI key
FZWBNHMXJMCXLU-UHFFFAOYSA-N
General description
Sephadex® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.
Application
Sephadex® G-50 has been used to purify the DNA products in cycle sequencing reactions.
Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.
Features and Benefits
- Excellent recovery
- Minimal sample dilution
- Quick and easy to use
Legal Information
Sephadex is a registered trademark of Cytiva
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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