SML2135
Biotinyl tyramide
≥97% (HPLC), powder, tyramide signal amplifier
Synonym(s):
(3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide, BP, Biotin-Phenol
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About This Item
Empirical Formula (Hill Notation):
C18H25N3O3S
CAS Number:
Molecular Weight:
363.47
UNSPSC Code:
12352200
NACRES:
NA.77
MDL number:
Assay:
≥97% (HPLC)
Form:
powder
Product Name
Biotinyl tyramide, ≥97% (HPLC)
assay
≥97% (HPLC)
form
powder
color
white to beige
solubility
DMSO: 2 mg/mL, clear
storage temp.
−20°C
SMILES string
O=C(NCCC1=CC=C(O)C=C1)CCCC[C@@H]2SC[C@@]3([H])[C@]2([H])NC(N3)=O
InChI
1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1
InChI key
VZWXNOBHWODXCW-ZOBUZTSGSA-N
Application
Biotinyl tyramide or Biotin-Phenol has been used in proximity-dependent labeling for protein identification.
Biochem/physiol Actions
Biotinyl tyramide is a reagent used for tyramide signal amplification for both immunohistochemistry (IHC) and in situ hybridization protocols and with either chromogenic or fluorescence detection. Preliminary binding of a probe is followed by secondary detection of the probe with an HRP-labeled antibody or streptavidin conjugate. Catalysis by the HRP results in the deposition of multiple biotinyl tyramide molecules in the immediate vicinity of the probe that can then be detected with a labeled streptavidin conjugate. Detection sensitivity can be 100-fold or more sensitive compared to conventional detection procedures.
Reagent used for tyramide signal amplification
Storage Class
11 - Combustible Solids
wgk
WGK 3
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| SML2135-250MG | 04061835502776 |
| SML2135-50MG | 04061835502783 |