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T9060

Sigma-Aldrich

BlueView™ Nucleic Acid Stain

in 10× TBE buffer, powder blend

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MDL number:
MFCD01634476

grade

for molecular biology

form

powder blend

detection sensitivity measuring range

<250 ng/band

storage temp.

room temp

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Application

BlueView is a quick, safe alternative to ethidium bromide staining for nucleic acid electrophoresis. BlueView can be used directly as the running buffer and in the gel for instant staining of the bands of nucleic acids during electrophoresis, visible in ambient light. Bands can be excised from the gel without the use of UV which can cause nicking. BlueView stained bands can be used for PCR, ligation, labeling, restriction digestion, and Southern blotting.

Reconstitution

Reconstitution with water produces a 10× stock solution of the BlueView stain in 10× TAE or TBE buffer. The bottles are large enough to contain the concentrate. Dilute the 10× stock solution to the desired concentration before use. Concentrations of TBE and TAE between 0.5 and 1× are commonly used for nucleic acid electrophoresis. A 20-fold dilution of the stock solution is recommended for low background staining and optimum signal to noise.

Legal Information

BlueView is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H360FD

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Thorunn Rafnar et al.
Nature genetics, 43(11), 1104-1107 (2011-10-04)
Ovarian cancer causes more deaths than any other gynecologic malignancy in developed countries. Sixteen million sequence variants, identified through whole-genome sequencing of 457 Icelanders, were imputed to 41,675 Icelanders genotyped using SNP chips, as well as to their relatives. Sequence

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