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L4516

Lipopolysaccharides from Escherichia coli O127:B8

Name: Lipopolysaccharides from <I>Escherichia coli</I> O127:B8
Brand: SIGMA

suitable for cell culture, BioXtra, γ-irradiated

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Synonym(s):

LPS

EC Number:

297-473-0

MDL number:

MFCD00212998

NACRES:

NA.75

Quality Level

200

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

purified by

gel-filtration chromatography

technique(s)

cell culture | mammalian: suitable

impurities

<1% Protein (Lowry)

solubility

H₂O: 5 mg/mL, slightly hazy

storage temp.

2-8°C

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Application

Lipopolysaccharides from Escherichia coli 0127:B8 has been used:

to study the LPS-induced macrophage survival in mice
to elicit the secretion of cytokines by cattle PBMC
to study apoptosis of highly susceptible macrophage cells in a LPS-induced proinflammatory environment
to perform autophagy assay in BV2 and BV2-LC3 cells
for stimulation of human monocytes, macrophages and mouse microglia cells
to induce pentraxin-3 expression in 293T cells

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4). Being an immunogenic antigen, it enhances immune responses to soluble antigens.

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Pictograms

GHS06

Signal Word

Danger

Hazard Statements

H300

Precautionary Statements

P264 - P270 - P301 + P310 - P405 - P501

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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