66825-U
BIOshell™ A400 Protein C4 (3.4 μm) HPLC Columns
L × I.D. 10 cm × 2.1 mm, HPLC Column
Synonym(s):
Protein C4 Reversed-Phase Chromatography Column
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About This Item
Product Name
BIOshell™ A400 Protein C4, 3.4 μm HPLC Column, 3.4 μm particle size, L × I.D. 10 cm × 2.1 mm
material
stainless steel hardware
Quality Level
agency
suitable for USP L26
description
Shell thickness (0.2 μm), Solid Core (3.0 μm)
product line
BIOshell™
feature
endcapped
manufacturer/tradename
BIOshell™
packaging
1 ea of
extent of labeling
0.4% carbon loading
parameter
600 bar max. pressure, 90 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable
L × I.D.
10 cm × 2.1 mm
surface area
15 m2/g
surface coverage
4.2 μmol/m2
matrix
spherical silica particle platform, superficially porous particle
matrix active group
C4 (butyl) phase
particle size
3.4 μm
pore size
400 Å
operating pH range
2-9
separation technique
reversed phase
General description
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Protocols
BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations
HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4
Larger porous shell particles in BIOshell™ columns improve efficiency in U/HPLC analysis of peptides and proteins.
Articles
BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.
The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
Related Content
生物分子是活细胞产生的大分子聚合物，作为活生物体的基本组成部分执行许多生命必需的生物过程。尽管现有很多分离和分析技术可供选择，最常用的还是高效液相色谱法(HPLC)。
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 66825-U | 04061833388143 |