Low pressure liquid chromatography

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Showing 1-26 of 26 results

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Comparison of Spherical and Irregular Silicas in Flash Chromatography

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protein G and protein A bind to different IgG

This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

Performing a purity and homogeneity check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from GE Healthcare.

Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Capto Adhere

Capto adhere from GE Healthcare is a multimodal strong anion exchanger for BioProcess applications.

Viral Clearance Using Capto™ Adhere

Description of viral clearance using Capto adhere from GE Healthcare.

Purification of Thiol-Containing Substances By Covalent Chromatography

Thiol-containing substances can be isolated selectively by covalent binding to an activated thiolated matrix via thiol-disulphide exchange to form a mixed disulphide bond. After washing away unbound material, the thiol-containing substance is eluted by reducing the disulphide bond. This technique

Troubleshooting Reversed Phase Chromatography (RPC)

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.

Protein Affinity Chromatography

Affinity chromatography is the process of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. This process allows for the highly specific and efficient purification of many diverse proteins and other compounds. The process requires

Bind/Elute vs Flowthrough Mode in Multimodal Chromatography

This page describes the difference between bind/elute and ﬂowthrough modes in multimodal chromatography using GE Healthcare products.

Ion Exchange Chromatography

Ion exchange chromatography is employed by the Genopure Plasmid Midi Kit and the Genopure Plasmid Maxi Kit.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Lewatit® Ion Exchange Resins – All-around Solutions for Chemical Processes

Chemical processes comprise the steps of processing chemical starting materials, synthesizing the products, isolating the generated materials from the reaction mixture and subsequent purification.

Optimization of Loading Conditions on Capto Adhere Using DoE

This page describes the optimization of loading conditions on Capto adhere from GE Healthcare using full factorial design of experiments (DoE).

LRA (Lipid Removal Agent) for Biopharmaceutical Production

Lipids are naturally-occurring hydrocarbons that are poorly soluble in water and soluble in non-polar solvents such as ether and chloroform.

Immobilized Metal Chelate Affinity Chromatography (IMAC)

This page shows how to separate proteins and peptides with afﬁnity for metal ions by immobilized metal chelate afﬁnity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from GE Healthcare.

Maintenance of Multimodal Chromatography Media and Storage Conditions

This page describes the maintenance of media and storage conditions for multimodal chromatography using GE Healthcare products.

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Purification or Removal of Viruses including Adeno-associated Virus

This page shows how to purify or remove viruses with a Capto DeVirS, AVB Sepharose High Performance from GE Healthcare.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

Capto MMC

Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.