Pharmacology and drug discovery research

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Chromatography Profile Analysis of Phosphorothioate Oligonucleotides

The ultimate goal of most antisense research is to develop therapeutic agents for diseases such as diabetes, cancer, and HIV/ AIDS. Phosphorothioate oligonucleotides (commonly referred to as S-oligos) are often the molecules of choice because of their resistance to cellular

Combining Permeability and Solubility Analyses in a Single Workflow

This article details the benefits of combining solubility and a non-cell based permeability assay (Parallel Artificial Membrane Permeation Assay; PAMPA) into a single workflow in which a portion of the filtrate from the MultiScreen® Solubility filter plate is added to

Phase I Drug Metabolism

Phase I biotransformation reactions introduce or expose functional groups on the drug with the goal of increasing the polarity of the compound. Although Phase I drug metabolism occurs in most tissues, the primary and first pass site of metabolism occurs

Assay and Organic Impurity Profiling of Apixaban Using an Ascentis^® Express C18 Column and UV Detection

Apixaban is sold under the tradename Eliquis and at present no pharmacopeial monographs are available for Axpixaban using either an assay and/or organic impurity profiling method. A newly developed and validated HPLC-UV method using a column with C18 solid core

Target Identification & Validation for Early Drug Discovery

Target identification, validation and characterization for early stages of drug discovery. Identifying drug target via genetic association, expression profiling.

Proteomic Ligandability Assessment

Ligandability describes the propensity of a protein target to bind a small molecule with high affinity. It is a precursor to evaluating druggability, which requires more advanced translational pharmacological effects and drug-like properties in vivo.

Immuno Oncology Research Tools and Solutions

Whether you need screening tools for CAR/TCR therapies and mAB development, target cells for efficacy testing, or potency assays for clinical development, we provide functional biologically-relevant solutions to help you save time, accelerate your immuno-oncology research, and simplify your workflow.

The Role of Liver Transporters in Drug-Drug Interactions

Oral drug delivery involves dissolution in the small intestine and absorption across the enterocyte barrier into the portal vein followed by subsequent delivery through the liver into the systemic circulation.

Antihypertensive Agents

Agents that inhibit angiontensin-converting enzymes (ACE) and angiotensin II formation are essential to cardiovascular medicine. These inhibitors are used not only to treat essential hypertension and complications associated with it, but also to prevent cardiovascular, cerebrovascular, and renal complications

Antimicrobial Peptides

With bacterial resistance and emerging infectious diseases becoming potential threats to humans, ribosomally synthesized antimicrobial peptides have become a promising focus area in antibiotic research.

Caco-2 Intestinal Drug Transporter Models for More Predictive Drug Absorption

Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.

Using human iPS Cell-derived Colon Organoids for Cytotoxicity Screening of Drug Compounds

Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.

Human Renal Proximal Tubule Epithelial Cells (RPTEC) as an Essential Advanced Model to Express Kidney Drug Transporters and Toxicity

Human renal proximal tubule epithelial cells (RPTECs) are commonly used to predict human renal drug permeability and to investigate drug efflux. We have generated transporter knockout (KO) cell lines using CompoZr® Zinc Finger Nuclease (ZFN) technology in a proprietary renal

LifeNet Health® Primary Human Hepatocytes

High viability cryoplateable and cryopreserved hepatocytes sourced from LifeNet Health®

Comparing Quantitative Viability Bioassays: An Evaluation of MTT, alamarBlue™, and Guava® ViaCount® Methods

This article shows a comparison of the quantitative viability bioassays MTT, alamarBlue™, and Guava® ViaCount®.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Primary Cell Culture Basics

What are primary cells? A comprehensive overview of animal and human primary cell lines and media and how they are used in biomedical cell culture applications. Primary cultures are less homogeneous relative to cell lines.

3D Organoid Culture: New In Vitro Models of Development and Disease

rganoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

Partial PROTACs for Targeted Protein Degradation

Partial PROTACs are a collection of crosslinker-E3 ligand conjugates with a pendant functional group for covalent linkage to a target ligand.

Unnatural Amino Acids

Unnatural amino acids, the non-proteinogenic amino acids that either occur naturally or are chemically synthesized, are becoming more and more important as tools for modern drug discovery research.

The Role of Intestinal Efflux Transporters In Drug Absorption

We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.

Drug transport assays in a 96-well system: reproducibility and correlation to human absorption

Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.

Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA)

This is a white paper on the Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA) using MultiScreen® Filter Plates.

Membrane Integrity Test for Lipid-PAMPA Artificial Membranes

This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes

Inhibition of Nucleic Acid Synthesis by Antibiotics

Quinolones are a key group of antibiotics that interfere with DNA synthesis by inhibiting topoisomerase, most frequently topoisomerase II (DNA gyrase), an enzyme involved in DNA replication.

Famciclovir Tablets USP Monograph Method Using a Purospher™ STAR RP-8 endcapped HPLC Column and UV Detection

Following the new USP monograph for Famciclovir Tablets, Purospher™ STAR RP-8 endcapped HPLC columns can be used to monitor organic impurities in Famciclovir tablet formulations. Famciclovir is an antiviral drug indicated for the treatment of herpes zoster, herpes simplex virus

Hit Discovery & Confirmation for Early Drug Discovery

Hit discovery and confirmation allows identification of molecules acting against the target. It requires compound screening assays, depending on the drug target.