Protein purification

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Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON®

This page describes manual purification of histidine-tagged proteins using His GraviTrap TALON from GE Healthcare.

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.

Performing a Purification of IgG Antibodies with Protein A HP SpinTrap™ columns

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein A HP SpinTrap columns from GE Healthcare.

Robust, load independent viral clearance in monoclonal antibody purification with NatriFlo® HD-Q membrane adsorber

Membrane adsorbers are a cost-effective, robust, single-use solution for monoclonal antibody polishing

Automated Purification of Antibodies Using ÄKTA Chromatography Systems

This page describes automated purification of antibodies using ÄKTA chromatography systems.

Agarose beads Vs. Magnetic beads in ChIP

Agarose beads Vs. Magnetic beads in Chromatin Immunoprecipitation (ChIP)

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify

Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.

Characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.

Characteristics of Dextrin Sepharose^® High Performance Products

This page shows the characteristics of Dextrin Sepharose High Performance products from GE Healthcare.

Protein G and protein A bind to different IgG

This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.

Troubleshooting Strategies in GST-tagged Protein Purification

This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.

Performing a Separation with IgG Sepharose 6 Fast Flow

This page shows how to perform a separation with IgG Sepharose 6 Fast Flow from GE Healthcare, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Chelators

BioUltra Chelators. A chelate is a water-soluble complex between a metal ion and a complexing agent. It usually does not dissociate easily in solution, but forms an inert complex.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Yeast Cell Lysis Reagents

Our yeast cell lysis reagents are versatile, phosphate-free, and non-denaturing. CelLytic Y Cell Lysis reagent provides an effective method for yeast cell lysis and protein solubilization. The CelLytic Y Yeast Enzymatic Lysis Kit provides a convenient method for

NEED TO DELETE THIS PAGE: Troubleshooting Puriﬁcation Methods

NEED TO DELETE THIS PAGE:

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by afﬁnity chromatography using HiTrap IgM Purification HP from GE Healthcare.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Performing a purity and homogeneity check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from GE Healthcare.

Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature

Performing an isolation of recombinant protein complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

Troubleshooting of Detection Methods of GST-tagged Proteins

Performing High-Throughput Screening Using His MultiTrap™ HP and His MultiTrap™ FF 96-Well ﬁlter Plates

This page shows high-throughput screening using His MultiTrap HP and His MultiTrap FF 96-well ﬁlter plates from GE Healthcare.

Amicon® Pro Purification System – Protein Purification

Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.

Performing a Purification of IgG Antibodies with Protein G GraviTrap™

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein G GraviTrap from GE Healthcare.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

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