Buffer Calculator

About Biological Buffers

Buffers are used in many types of scientific studies to maintain or control the acidity of a solution within a desired physiological range, or even to initiate a particular reaction. The pH of substances affects both the rate and efficiency of chemical reactions, and the recovery and purity of products. Hence, buffers considerably contribute to variations in the outcome, and ultimately the success or failure, of studies. Therefore, understanding buffers and error-free calculation of buffers is essential to reduce variability and undesired interactions for reliable research.

Using the buffer preparation calculator

This buffer calculator provides an easy-to-use tool to calculate buffer molarity and prepare buffer solutions using the formula weight of the reagent and your desired volume (L, mL, or µL) and concentration (M, mM, or nM).

To calculate the amount of buffer needed, please select a buffer from the Selection menu. The empirical formula, pKa, buffer pH range, formula weight and product list will appear. Enter the desired final volume and concentration and click “Calculate Mass.” The exact mass of the buffer will be calculated in grams and a step-by-step buffer recipe is automatically provided to assist in buffer preparation.

Click on the list of product results for detailed information on our available buffers and to place an order.