Merck
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  • Design of a cellular-uptake-shielding magnetic catcher for cancer cell separation.

Design of a cellular-uptake-shielding magnetic catcher for cancer cell separation.

Macromolecular bioscience (2012-09-12)
Ya Wang, Jing Zhang, Hui-Zhen Jia, Juan Yang, Si-Yong Qin, Chen-Wei Liu, Ren-Xi Zhuo, Xian-Zheng Zhang
ABSTRACT

Fluorescent-magnetic-biotargeting multifunctional microcapsules (FMBMMs) are designed and fabricated via layer-by-layer assembly. It is found that the arginine-glycine-aspartate-modified FMBMMs were capable of sensitively detecting and efficiently isolating approximately 80% target cancer cells within 20 min. More importantly, FMBMMs present a general template for identifying and separating multiple types of cancer cells simply by altering the recognition motif.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poly(allylamine) solution, 20 wt. % in H2O
Sigma-Aldrich
Poly(allylamine) solution, average Mw ~65,000, 10 wt. % in H2O
Sigma-Aldrich
Poly(allylamine hydrochloride), average Mw ~17,500 (GPC vs. PEG std.)
Sigma-Aldrich
Poly(allylamine hydrochloride), average Mw 50,000