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Column	Ascentis Express OH5, 10 cm x 3.0 mm I.D., 2.7 µm particles (53769-U)
Column temp.	35 °C
Mobile phase	2 mM ammonium acetate in 10:90 water:acetonitrile, pH to 7.0 with acetic acid, glacial
Flow rate	0.5 mL/min
Pressure	850 psi (59 bar)
Sample	100 µg/mL salicylic acid, 200 µg/mL acetylsalicylic acid in methanol:acetonitrile (3:7)
Injection	2 µL
Detector	UV, 235 nm

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Categories	Analytical Chromatography, Analgesics
Featured Industry	Pharmaceutical (small molecule)
Legal Information	Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Suitability	Application for HPLC

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