The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.7)
One unit causes the formation of one milligram of reducing sugars equivalent to the glucose at 3 minutes under the conditions described below (This activity is equivalent to the international unit that hydrolyzes one micromole of saccharose per minute at the same temperature).
Reagents
Concentration in assay mixture | |
Acetate buffer | 65 mM |
Saccharose | 73 mM |
* Dissolve the enzyme preparation in ice-cold distilled water and dilute to 2.0-9.0 U/mL with the enzyme dilute (H), immediately before assay.
Activity can be calculated by using the following formula:
Weight activity (U/mg)=(U/mL)×1/C
This procedure is for informational purposes. For a current copy of our quality control procedure, please contact our Technical Service Department.
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