Home QuEChERS Sample Preparation MethodDetermination of Neonicotinoids in Honey using a Chromolith RP-18 HPLC column and UV Detection

Determination of Neonicotinoids in Honey using a Chromolith RP-18 HPLC column and UV Detection

Anita Piper

Life Science Application Centre (GAC), Darmstadt, Germany

Introduction

Neonicotinoids are water soluble insecticides. They are used for crop protection and can migrate from treated soil into plant tissue and flower nectar. Neonicotinoids have a negative effect on brain cells of honey bees, and colony collapse can occur. [Clothianidin, Imidacloprid and Thiamethoxam are banned in Germany, (EU) No. 485/2013].

This application focuses on HPLC testing of neonicotinoids in honey. The limit of detection (LOD) and limit of quantitation (LOQ) for neonicotinoids is better than 30 µM and 90 µM, respectively, when using HPLC combined with UV detection.

Determination of Neonicotinoids in Honey

Chromatographic Data - blank run

Chromatographic Data - Standard Solution

Standard Solution (200 µM @ 254 nm)

Standard Solution (200 µM @ 270 nm)

Chromatographic Data - Sample Solution spiked

Sample Solution Spiked @ 254 nm

Sample Solution @ 270 nm

Chromatographic Data - Sample Solution spiked

Sample Solution Spiked @ 254 nm

Sample Solution Spiked @ 270 nm

1. Specificity: Inject standard solution of neonicotinoids (200 µM) and determine the retention time and content of desired analyte @254 nm

1. Specificity: Inject standard solution of neonicotinoids (200 µM) and determine the retention time and content of desired analyte @270 nm

2. Standard repeatability honey (spiked with 5 µL of a 200 µM standard solution) in mAU*min

3. LOD & LOQ

4. Linearity (area mAU*min)

Dinutefuran @270 nm

Nitenpyram @270 nm

Thiamethoxam @254 nm

Imidacloprid @270 nm

Clothianidin @270 nm

Acetamiprid @254 nm

Thiacloprid @254 nm

Materials List

Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?

Determination of Neonicotinoids in Honey using a Chromolith RP-18 HPLC column and UV Detection

Introduction

Chromatographic Data - blank run

Chromatographic Data - Standard Solution

Standard Solution (200 µM @ 270 nm)

Chromatographic Data - Sample Solution spiked

Sample Solution @ 270 nm

Chromatographic Data - Sample Solution spiked

Sample Solution Spiked @ 270 nm

1. Specificity: Inject standard solution of neonicotinoids (200 µM) and determine the retention time and content of desired analyte @254 nm

1. Specificity: Inject standard solution of neonicotinoids (200 µM) and determine the retention time and content of desired analyte @270 nm

2. Standard repeatability honey (spiked with 5 µL of a 200 µM standard solution) in mAU*min

3. LOD & LOQ

4. Linearity (area mAU*min)

Dinutefuran @270 nm

Nitenpyram @270 nm

Thiamethoxam @254 nm

Imidacloprid @270 nm

Clothianidin @270 nm

Acetamiprid @254 nm

Thiacloprid @254 nm