Anti-SHP-1/2 Antibody, clone NL213, rabbit monoclonal

~65kDa

Full-length glutathione transferase SHP-1 fusion protein, purified from bacteria

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

This Anti-SHP-1/2 Antibody, clone NL213 is validated for use in IP, WB for the detection of SHP-1/2.

Others not tested.

Recognizes SHP-1 greater than SHP-2, Mr ~65 kDa

Cultured supernantant in 0.05% sodium azide

Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot in RIPA lysates from human A431 carcinoma cells

Replaces: 05-742

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