Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal

Mr 22kDa

Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganisation, and membrane trafficking.

KLH-conjugated, synthetic peptide (SYPDTDVILMCFSIDSPDSLENKK) corresponding to amino acids 73-94 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

This Anti-Rho (-A Antibody, -B, -C), clone 3L74 is validated for use in WB for the detection of Rho (-A, -B, -C).

Western Blot Analysis:
A 1:1000-1:4000 dilution of this lot detected Rho (-A, -B, -C) in RIPA lysates from human HL-60 and rat PC-12 cells. This antibody also recognized recombinant RhoA protein, but not recombinant rac1 or cdc42 proteins.

Broad species cross-reactivity expected based on sequence homology

Recognizes Rho (-A, -B, -C)

Cultured supernantant in 0.05% sodium azide

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

routinely evaluated by immunoblot on modified RIPA lysates from HL-60 cells

Replaces: 05-822

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