05-1336
Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312
clone CMA312, from mouse
别名:
H3S10P, Histone H3 (phospho S10), H3 histone, family 3A
登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
CMA312, monoclonal
Species reactivity:
vertebrates, human
Application:
ChIP, ELISA, ICC, WB, multiplexing
Citations:
12
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
CMA312, monoclonal
species reactivity
vertebrates, human
technique(s)
ChIP: suitable, ELISA: suitable, immunocytochemistry: suitable, multiplexing: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer10)
Gene Information
human ... H3C1(8350)
General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312 is a mouse monoclonal antibody for detection of phospho-Histone H3 (Ser10) also known as H3S10P, Histone H3 (phospho S10) & has been validated in WB, ELISA, ICC, Multiplexing and ChIP.
~17 kDa
Application
ELISA: This antibody has been shown by an outside laboratory to be suitable for ELISA.1
Immunocytochemistry: This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Multiplexing:
This antibody specifically recognizes histone H3 phosphorylated on Ser10 by Luminex® assay.
Immunocytochemistry: This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Multiplexing:
This antibody specifically recognizes histone H3 phosphorylated on Ser10 by Luminex® assay.
Biochem/physiol Actions
Broad species cross-reactivity is expected, based on sequence homology.
Physical form
Format: Purified
Analysis Note
Western Blot Analysis: 1 μg/mL of this antibody detected phospho-Histone H3 (Ser10) on 1 μg of colcemid-treated HeLa acid extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1093
Legal Information
Luminex is a registered trademark of Luminex Corp
Still not finding the right product?
试用我们的 产品选型工具 工具缩小选择范围
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
12 - Non Combustible Liquids
Hong Liu et al.
Nature cell biology, 15(1), 40-49 (2012-12-18)
Timely dissolution of sister-chromatid cohesion in mitosis ensures accurate chromosome segregation to guard against aneuploidy and tumorigenesis. The complex of shugoshin and protein phosphatase 2A (SGO1-PP2A) protects cohesin at centromeres from premature removal by mitotic kinases and WAPL in prophase.
Manjit Singh et al.
The Plant cell, 23(2), 443-458 (2011-02-18)
Apomixis is a form of asexual reproduction through seeds in angiosperms. Apomictic plants bypass meiosis and fertilization, developing offspring that are genetically identical to their mother. In a genetic screen for maize (Zea mays) mutants mimicking aspects of apomixis, we
Mitotic arrest and apoptosis in breast cancer cells induced by Origanum majorana extract: upregulation of TNF-? and downregulation of survivin and mutant p53.
Al Dhaheri, Y; Eid, A; AbuQamar, S; Attoub, S; Khasawneh, M; Aiche, G; Hisaindee, S; Iratni, R
Testing null