16-316
抗磷酸酪氨酸抗体，4G10® 铂，HRP结合物
Upstate®, from mouse, peroxidase conjugate
别名:
phosphotyrosine
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
peroxidase conjugate
Clone:
4G10®, monoclonal, PY20, monoclonal
Application:
ELISA, WB
Citations:
8
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody product type
primary antibodies
clone
4G10®, monoclonal, PY20, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable, western blot: suitable
isotype
IgG2b
shipped in
wet ice
target post-translational modification
phosphorylation (pTyr)
Gene Information
human ... PID1(55022)
General description
1989年抗磷酸酪氨酸克隆4G10的开发对研究人员来说是一个巨大的发现。4G10是第一个检测酪氨酸磷酸化的单克隆抗体，是检测酪氨酸磷酸化的最佳单克隆抗体。4G10以其灵敏度和检测多种底物上多种酪氨酸磷酸化的能力而闻名。在过去的20年中，它已经在几乎每个应用和酪氨酸靶标中得到了数千名科学和医学研究人员的验证。为了改进一百种尝试过但没有人成功的东西，我们将4G10 与下一个最受推崇的抗磷酸酪氨酸克隆PY20合并在一起，制成4G10 铂。PY20本身是4G10的非常差的替代品，但是它的累加效应可在更多的底物上进行更高水平的检测，甚至可以单独使用4G10 。
变化
Application
使用抗磷酸酪氨酸抗体，4G10铂，HRP偶联物（小鼠单克隆抗体）检测磷酸酪氨酸，该抗体已在WB，ELISA中显示有效。
Physical form
100 μL经蛋白G纯化的小鼠单克隆4G10（IgG2bƙ）HRP偶联物和经蛋白A纯化的小鼠单克隆PY20，HRP偶联物（IgG2b）的专有混合物，溶于含有0.05% Kathon和HRP稳定化合物的PBS中。如果在10 mL封闭缓冲液中以1:2000 – 1:4000的最佳浓度使用，则足以进行最少10次免疫印迹。 液体。
Analysis Note
通过蛋白质印迹法进行了常规评估。
Legal Information
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Hazard Classifications
Aquatic Chronic 2 - Skin Sens. 1
wgk
WGK 3
flash_point_f
does not flash
flash_point_c
does not flash
Ondřej Ballek et al.
Immunology letters, 142(1-2), 64-74 (2012-01-28)
Lck is the principal signal-generating tyrosine kinase of the T cell activation mechanism. We have previously demonstrated that induced Lck activation outside of lipid rafts (LR) results in the rapid translocation of a fraction of Lck to LR. While this
Wei Tian et al.
BMC biochemistry, 13, 22-22 (2012-10-25)
Phosphatase of regenerating liver-3 (PRL-3 or PTP4A3) has been implicated in controlling cancer cell proliferation, motility, metastasis, and angiogenesis. Deregulated expression of PRL-3 is highly correlated with cancer progression and predicts poor survival. Although PRL-3 was categorized as a tyrosine
Identification of a novel EphB4 phosphodegron regulated by the autocrine IGFII/IRA axis in malignant mesothelioma.
Scalia, et al.
Oncogene, 38, 5987-6001 (2021)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 16-316 | 04053252729423 |
| Z261580-1EA | 04061832530970 |
| Z261599-1EA | 04061832530987 |
| Z261610-1EA | 04061832530994 |
| Z261629-1EA | 04061832531007 |