Quality Level
manufacturer/tradename
EZ-ChIP
technique(s)
immunoprecipitation (IP): suitable
shipped in
wet ice
General description
用于富集染色质的特定区域,该区域可以用染色质蛋白的特异性抗体进行免疫沉淀。适用于所有真核细胞类型。 研究人员必须凭经验确定检测目的基因或启动子的染色质免疫沉淀反应。建议使用定量 PCR 检测。
Packaging
试剂盒容量:22 次测定
Physical form
包含进行 22 次独立的染色质免疫沉淀(ChIP)反应所需的所有试剂。 提供的缓冲液足以从多达五个 15 cm 平板的培养细胞上产生染色质,每个平板可提供多达 10 个染色质制剂(随细胞和测定类型的不同而不同)。
Analysis Note
通过使用抗乙酰基组蛋白 H4 多克隆抗体(06-866)免疫沉淀乙酰化的组蛋白 H4 进行了常规评估。 随后使用相同的抗体进行了免疫印迹分析。
Other Notes
蛋白 A 琼脂糖/鲑鱼精子 DNA(货号16-157)
所有必需的缓冲液
所有必需的缓冲液
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
target_organs
Respiratory Tract
wgk
WGK 3
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 2 - ED ENV 1 - Eye Irrit. 2 - STOT RE 2 Inhalation
相关内容
蛋白质是细胞内部的主要功能分子,负责细胞功能和生存的多种生物学活动。值得关注的是,许多蛋白质也能与DNA发生相互作用。
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
Regulation of human papillomavirus type 31 gene expression during the differentiation-dependent life cycle through histone modifications and transcription factor binding.
Tonia R Wooldridge, Laimonis A Laimins
Virology null
Distribution of acetylated histones resulting from Gal4-VP16 recruitment of SAGA and NuA4 complexes.
M Vignali et al.
The EMBO journal, 19(11), 2629-2640 (2000-06-03)
We analyzed the targeting of histone acetyltransferase (HAT) complexes by DNA-binding activators during transcriptional activation and the resulting distribution of acetylated histones. An in vitro competition assay was developed to acetylate and transcribe a nucleosomal array template in the presence
Seed-based systematic discovery of specific transcription factor target genes.
Mrowka, R; Bluthgen, N; Fahling, M
FEBS Journal null
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 17-295 | 08436037124679 |


