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PICM0RG50

Millicell器官培养型站立式培养小室, 亲水PTFE膜, 0.4 µm, 30 mm, 50/pk

pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 5 mm, sterile

别名:

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm

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关于此项目

UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32011202
Material:
transparent PTFE membrane, polystyrene housing (5 mm height)
Pore size:
0.4 μm
Sterility:
ethylene oxide treated, sterile
Binding type:
low binding surface
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material

transparent PTFE membrane, polystyrene housing (5 mm height)

sterility

ethylene oxide treated, sterile

feature

hydrophilic

manufacturer/tradename

Millicell®

packaging

pkg of Individually blister packaged

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable, cell differentiation: suitable

H

5 mm

diam.

30 mm

size

6 wells

surface area

4.2 cm2

color

transparent membrane, when wetted

matrix

Biopore™

pore size

0.4 μm

binding type

low binding surface

detection method

fluorometric

shipped in

ambient

storage temp.

10-30°C

General description

凭借Millicell嵌套板，附着或悬浮细胞可以从其上下两侧接触到培养基。更加密切地模拟了体内所发生的细胞生长、结构和功能。另外，Millicell嵌套板可用于从两侧研究单层细胞。

Application

  • 细胞附着、3D 细胞培养、细胞生长、细胞分化、免疫细胞化学
  • Biopore 膜（亲水PTFE）是低蛋白结合、活细胞观察和免疫荧光应用的理想选择。

Features and Benefits

  • 三维外植体结构的高活力和优越性研究
  • 较低的高度令其适合装入标准培养皿
  • Biopore (PTFE) 膜提供高活力—长达40天—和良好的跨膜氧转运
  • 该膜是透明的，并针对器官型外植体的长期维护进行优化。

Preparation Note

为确保膜从基底侧充分润湿，并减少气泡形成的几率，请按照以下步骤操作：
  1. 向接收孔中加入检测溶液或培养基：对于6孔板格式或等效尺寸，加入2 mL。
  2. 使用镊子以30°-45°的角度将插件放入接收孔中，以从基底侧逐渐润湿膜。
  3. 当插件边缘开始润湿时，轻轻放下插件，直至其完全嵌入孔中。

Legal Information

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10-13 - German Storage Class 10 to 13

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实验方案

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Toluidine Blue Staining Protocol for Millicell® Inserts

Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.

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全球贸易项目编号

货号GTIN
PICM0RG5004053252360763