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Merck
CN

AB3832

Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific

Chemicon®, from rabbit

别名:

Anti-EBP50, Anti-NHERF, Anti-NHERF-1, Anti-NHERF1

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
monkey, human, mouse, rat
Application:
IHC, WB
Citations:
1
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biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

monkey, human, mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pThr567)

Gene Information

human ... SLC9A3R1(9368)

General description

75KDa Moesin, and ~80kDa Ezrin & Radixin

Immunogen

Epitope: phospho-specific
KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Thr567 of human ezrin

Application

Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific detects level of Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) & has been published & validated for use in IH(P) & WB.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Western blotting 1:1,000 (for best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight). The antibody recognizes 75KDa (Moesin, and ~80kDa Ezrln & Radixin) bands.

Immunohistochemistry (paraffin): 1:50

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Thr567 phosphorylated ezrin, Thr564 phosphorylated radixin, and Thr558 phosphorylated moesin. Antibody does not cross-react with other related phospho proteins such as merlin or band 4.1.

Physical form

ImmunoAffinity Purified
Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/ml BSA, with 50% glycerol.

Preparation Note

Maintain at -20°C for 12 months, do not aliquot.

Analysis Note

Control
Cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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存储类别

10 - Combustible liquids

wgk

WGK 2



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Minji Kim et al.
Journal of cell science, 128(23), 4317-4327 (2015-10-21)
Tubulogenesis is fundamental to the development of many epithelial organs. Although lumen formation in cysts has received considerable attention, less is known about lumenogenesis in tubes. Here, we utilized tubulogenesis induced by hepatocyte growth factor (HGF) in MDCK cells, which



全球贸易项目编号

货号GTIN
AB383204053252279188