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Merck
CN

AB9750

Anti-14-3-3 phospho Serine58 Antibody

Chemicon®, from rabbit

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
rat, human
Application:
WB
Citations:
10
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biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pSer58)

Gene Information

human ... YWHAE(7531)

Immunogen

Synthetic peptide of amino acids surrounding the phosphoSerine 58 site of rat 14-3-3 Protein.

Application

Anti-14-3-3 phospho Serine58 Antibody detects level of 14-3-3 phospho Serine58 & has been published & validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western blot: 1:1,000

Biochem/physiol Actions

14-3-3 Protein, phosphoSerine58. The antibody recognizes a protein of ~29 kDa corresponding to 14-3-3 Protein, phosphoSerine58 in lysates from rat brainstem. Immunolabeling is blocked by the phosphopeptide used as the immunogen but not the corresponding non-phosphopeptide.
The immunogen sequence has 100% conservation with mouse and Xenopus.

Physical form

ImmunoAffinity Purified
Liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Preparation Note

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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存储类别

10 - Combustible liquids

wgk

WGK 2



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14-3-3 proteins in the nervous system
Berg, Daniela, et al
Nature Reviews. Neuroscience, 4, 752-762 (2003)
Joanna M Woodcock et al.
The Journal of biological chemistry, 278(38), 36323-36327 (2003-07-17)
The 14-3-3 proteins play a central role in the regulation of cell growth, cycling, and apoptosis by modulating the functional activities of key signaling proteins. Through binding to a phosphoserine motif, 14-3-3 alters target proteins activities by sequestering them, relocalizing
14-3-3 proteins: a number of functions for a numbered protein
Bridges, D. and Moorhead, G.B.G.
Science's STKE : Signal Transduction Knowledge Environment, 2005, re10-re10 (2005)



全球贸易项目编号

货号GTIN
AB975004053252675447