biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
AE5, monoclonal
species reactivity
human, mouse, bovine, rabbit
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KRT3(3850)
General description
64 kDa
Immunogen
兔角膜上皮角蛋白
Application
使用冷冻切片进行的免疫组化:1:50。
使用石蜡包埋切片进行的免疫组化:在胰蛋白酶消化抗原修复后,将石蜡包埋的组织切片按1:50比例固定在10%中性缓冲福尔马林或Fekete’s酸-酒精-福尔马林中(Mikaelian et al.,2004)。
蛋白质印迹:1:500。
最佳工作稀释度必须由最终用户进行确定。
使用石蜡包埋切片进行的免疫组化:在胰蛋白酶消化抗原修复后,将石蜡包埋的组织切片按1:50比例固定在10%中性缓冲福尔马林或Fekete’s酸-酒精-福尔马林中(Mikaelian et al.,2004)。
蛋白质印迹:1:500。
最佳工作稀释度必须由最终用户进行确定。
抗角蛋白K3/K76抗体,克隆AE5是抗角蛋白K3/K76的抗体,用于WB,IH,IH(P)。
Biochem/physiol Actions
该抗体与角膜上皮的64 kDa多肽(细胞角蛋白3;角蛋白K3)和上颚上皮的角蛋白K76(细胞角蛋白K2p)反应。 因此,这代表了研究角膜上皮类型分化的极佳标记。也可识别牛口鼻和唇上皮以及兔角膜细胞系。
Physical form
形式:纯化
通过离子交换色谱法纯化单克隆抗体,将其置于含有0.09%叠氮化钠的磷酸盐缓冲生理盐水(pH 7.2)中。
Preparation Note
若购买后1个月内使用,则储存于2°-8°C,若要长期储存,则将抗体等分成小体积并储存于-20°C,保存达1年。
Other Notes
浓度:请参考批次特异性浓缩物的检验报告。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Still not finding the right product?
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
12 - Non Combustible Liquids
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| CBL218 | 04053252361777 |