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Merck
CN

MABC965

Anti-Prohibitin-1 (PHB1), clone 1G5C1 Antibody

clone 1G5C1, from rat

别名:

Prohibitin

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1G5C1, monoclonal
Application:
IHC, WB
Citations:
2
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biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1G5C1, monoclonal

species reactivity

human, mouse, rat

technique(s)

immunohistochemistry: suitable, western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PHB(5245)

General description

Prohibitin is an inhibitor of DNA synthesis that is widely expressed across various tissues. Prohibitin influences proliferation, and may also have a role in regulating mitochondrial respiration activity and in aging. Expression levels of prohibitin in fibroblasts have been found to decrease heterogeneously as cellular aging occurs.
~30 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human Prohibitin-1 (PHB1).

Application

Anti-Prohibitin-1 (PHB1), clone 1G5C1 | MABC965 is an antibody against Prohibitin-1 for use in Western Blotting, Immunohistochemistry.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Prohibitin-1 (PHB1) in 10 µg of NIH/3T3 and HeLa cell lysate.
Immunohistochemistry Analysis: A representative lot detected Prohibitin-1 (PHB1) in rat liver tissue (Kakehashi, A., et al. (2011). Toxicological Sciences. 119(1):61-72).

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in rat liver tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Prohibitin-1 (PHB1) in 10 µg of rat liver tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Anna Kakehashi et al.
Toxicological sciences : an official journal of the Society of Toxicology, 119(1), 61-72 (2010-10-12)
In the present study, protein lysates from microdissected glutathione S-transferase placental-form-positive (GST-P(+)) foci and hepatocellular carcinomas from livers of rats treated with N-diethylnitrosamine followed by phenobarbital at doses of 0 and 500 ppm in the diet for 10 and 33



全球贸易项目编号

货号GTIN
MABC96504055977125290