form
lyophilized (salt-free)
specific activity
20 U/mg
mol wt
30 kDa
packaging
pkg of 2 mg
manufacturer/tradename
Roche
optimum pH
8.0-8.5
General description
25℃，以Z-Phe-Leu-Glu-4-硝基苯胺为底物（37℃，以酪蛋白为底物，冻干物约500 U/mg）。
内切蛋白酶Glu-C是一种葡萄球菌丝氨酸蛋白酶。其抑制剂包括DFP、α2-巨球蛋白、以及TLCK。
在+25°C下，以Z-Phe-Leu-Glu-4-硝基苯胺为底物（+37°C，以酪蛋白为底物，冻干物约500 U/mg）时，冻干物酶活性约为20 U/mg。
Application
使用内切蛋白酶Glu-C（V8蛋白酶）进行蛋白结构分析和序列分析。
Biochem/physiol Actions
热灭活：通过煮沸10分钟使内切蛋白酶Glu-C失活。
内切蛋白酶Glu-C可特异性地水解Glu（或同时水解Glu和Asp）羧基侧的肽和酯键，其取决于所用的缓冲液。
Preparation Note
激活剂：该酶在SH试剂存在下具有最大活性
工作浓度：1 至 5 mM
工作溶液: 推荐的溶剂是50mM乙酸铵（pH 4.0，2mg/ml）。
储存条件（工作溶液）：-15至-25°C
分液冷冻保存并仅解冻一次，酶（2mg/ml，溶于50mM乙酸铵中，pH 4.0）可稳定保存至少一个月。
工作浓度：1 至 5 mM
工作溶液: 推荐的溶剂是50mM乙酸铵（pH 4.0，2mg/ml）。
储存条件（工作溶液）：-15至-25°C
分液冷冻保存并仅解冻一次，酶（2mg/ml，溶于50mM乙酸铵中，pH 4.0）可稳定保存至少一个月。
在2°C至8°条件下储存。 （请保持干燥！）
Other Notes
仅用于生命科学研究。不可用于诊断。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
此项目有
Rosalynn C Molden et al.
Current protocols in protein science, 77, 23-23 (2014-08-02)
Histones are chromatin proteins that are highly modified with many different types of post-translational modifications. These modifications act in concert to regulate a number of chromatin-related processes. However, identification and quantification of co-occurring histone post-translational modifications is challenging because there
Marion Avril et al.
PLoS pathogens, 9(6), e1003430-e1003430 (2013-07-05)
During blood stage infection, Plasmodium falciparum infected erythrocytes (IE) bind to host blood vessels. This virulence determinant enables parasites to evade spleen-dependent killing mechanisms, but paradoxically in some cases may reduce parasite fitness by killing the host. Adhesion of infected
Tianshi Wang et al.
Molecular cell, 75(4), 823-834 (2019-07-16)
Sirt3, as a major mitochondrial nicotinamide adenine dinucleotide (NAD)-dependent deacetylase, is required for mitochondrial metabolic adaption to various stresses. However, how to regulate Sirt3 activity responding to metabolic stress remains largely unknown. Here, we report Sirt3 as a SUMOylated protein in
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 10791156001 | 04061838676719 |