Merck
CN

11681451001

Roche

NBT/BCIP 储存液

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别名:
5-溴-4-氯-3-吲哚磷酸, BCIP

形式

solution

质量水平

分子量

817.7

包装

pkg of 8 mL

制造商/商品名称

Roche

储存温度

2-8°C

一般描述

反应产物呈蓝色,不溶于水。蓝色为肉眼可见。

配方:NBT:C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N

评估: 肉眼

应用

  • 该溶液用于通过印迹方案灵敏检测碱性磷酸酶(AP),适用于:DNA印迹
  • RNA印迹
  • 蛋白质印迹
  • 菌落及菌斑清除
  • 原位杂交
  • 以及免疫组织化学和免疫化学应用
注: NBT/BCIP可同时用于硝酸纤维膜和尼龙膜。

外形

溶液中含硝基蓝四唑氯铵18.75 mg/ml, 5-溴-4-氯-3-吲哚基磷酸盐9.4 mg/ml,甲苯胺盐67% (DMSO) (v/v)。
注: 溶液颜色可能从黄色到淡棕色不等。

制备说明

工作溶液:制备染色液
该溶液需临使用前配制。
  • 除DIG体系外的其他应用:向10 ml的 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2中加入200 μl的储备溶液。
  • DIG体系应用:向10 ml的 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl中加入200 μl的储备溶液
注:检测缓冲液中不能含MgCl2,否则会导致检测操作步骤后在膜上呈现斑点背景。不溶于水。

其他说明

仅用于生命科学研究。不可用于诊断。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

does not flash

闪点(°C)

does not flash


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Morgan Haugen et al.
Cold Spring Harbor protocols, 2010(10), pdb-pdb (2010-10-05)
Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have
Jaroslav Fabik et al.
Biology open, 9(6) (2020-07-04)
Cranial neural crest cells (cNCCs) originate in the anterior neural tube and populate pharyngeal arches in which they contribute to formation of bone and cartilage. This cell population also provides molecular signals for the development of tissues of non-neural crest
Rebecca McLennan et al.
Developmental biology, 339(1), 114-125 (2009-12-29)
The neural crest is an excellent model to study embryonic cell migration, since cell behaviors can be studied in vivo with advanced optical imaging and molecular intervention. What is unclear is how molecular signals direct neural crest cell (NCC) migration
Thomas S Klaric et al.
Stem cell research & therapy, 5(3), 64-64 (2014-06-03)
Npas4 is a calcium-dependent transcription factor expressed within neurons of the brain where it regulates the expression of several genes that are important for neuronal survival and synaptic plasticity. It is known that in the adult brain Npas4 plays an
Dany Gaillard et al.
PLoS genetics, 11(5), e1005208-e1005208 (2015-05-29)
Continuous taste bud cell renewal is essential to maintain taste function in adults; however, the molecular mechanisms that regulate taste cell turnover are unknown. Using inducible Cre-lox technology, we show that activation of β-catenin signaling in multipotent lingual epithelial progenitors

实验方案

NBT/BCIP signals should not be mounted with xylene containing mounting media (e.g., DPX), because these could lead to crystal formations of the color precipitates. Classical counterstains, such as eosin, however, require xylene containing mounting media.

NBT/BCIP Stock Solution Protocol & Troubleshooting

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