usage
mL sufficient for 100 mini-gels
Quality Level
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
concentration
10,000 × in DMSO
greener alternative category
storage temp.
−20°C
General description
SYBR® Green II是用于琼脂糖或聚丙烯酰胺凝胶中RNA和ssDNA电泳后染色的一种高度敏感染料。SYBR® Green II对RNA染色没有选择性,但与双链DNA结合时相比较,与RNA结合时确实显示出更高的量子产率。
默克生命科学致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。与溴化乙锭染色的标准用途相比,该产品具有固有的更安全的化学特性。如需更多信息,请参阅可在相关内容中找到的文献。
Application
SYBR® Green II RNA可用于:
- 大肠杆菌(Escherichia coli)核糖核酸(RNA)定量分析
- 在Northern blot印迹分析前,对聚丙烯酰胺凝胶分离的5′-ETS rRNA进行染色
- 染色甲醛琼脂糖凝胶,使RNA条带显色
Features and Benefits
- 用于琼脂糖或聚丙烯酰胺凝胶中RNA和ssDNA电泳后染色的超灵敏染色剂
- 497 nm下激发最大,254nm处还有次级激发峰
- SYBR®Green II染色RNA的荧光发射波长为520 nm
- 使用SYBR Green II对琼脂糖/甲醛凝胶进行染色不会干扰RNA转移至细胞膜或Northern印迹分析中的后续杂交,只要预杂交和杂交缓冲液内包含0.1%-0.3% SDS用于去除染料。
- 适合类病毒RNA和细胞多拷贝RNA检测
- 在单链构象多态性(SSCP)分析等要求极高灵敏度的检测方法中,灵敏度超过溴化乙锭
Legal Information
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
wgk
WGK 3
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
相关内容
Nancy-520 for DNA Detection and Quantitation
Human ??defensin-1 and -2 expression in the gingiva of patients with specific periodontal diseases
S. Vardar-Sengul
Journal of Periodontal Research (2007)
Dyes for in vitro detection of nucleic acids and proteins.
BioProbes, 20, 12-13 (1994)
L G Lee et al.
Cytometry, 7(6), 508-517 (1986-11-01)
The purpose of this study was to find a 488-nm excitable fluorescent dye for reticulocyte analysis by the use of fluorescence activated cell cytometry. The chemical structure of thioflavin T, a dye used for reticulocyte analysis with 457-nm excitation, was
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| S9305-.5ML | 04061836961329 |