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文件

P3143

Supelco

聚乙烯亚胺 溶液

analytical standard, 50 % (w/v) in H2O

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别名:
PEI, 乙烯亚胺聚合物 溶液
CAS号:
9002-98-6
PubChem化学物质编号:
24898396

等级

analytical standard

质量水平

100

分子量

Mn ~60,000
Mw 750,000

浓度

50 % (w/v) in H2O

技术

gel permeation chromatography (GPC): suitable

格式

single component solution

InChI

1S/C2H5N/c1-2-3-1/h3H,1-2H2

InChI key

NOWKCMXCCJGMRR-UHFFFAOYSA-N

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相关类别

应用

聚乙烯亚胺是一种水溶性聚合物，用于过滤冷冻细胞膜的膜润滑剂。小柱中的PEI纤维素将从相关化合物中分离环核苷酸，其电解质浓度与在PEI纤维素涂层板上进行薄层色谱时所用的电解质浓度相同。PEI溶液还可用于合成纳米纤维膜，即吸附废水样品中的阴离子染料和重金属离子。

其他说明

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

象形图

Exclamation markEnvironment
GHS07,GHS09

警示用语：

Warning

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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示例

T1503
货号
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包装规格/数量

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the molar concentration of Product P3143, Poly(ethyleneimine) solution?

    This product is nominally a 50% w/v solution of PEI in water, meaning 50 grams of poly(ethyleneimine) per 100 mL of solution. Concentrations of polymer solutions (such as PEI) are commonly reported either as a percentage or mg/mL, not in terms of molarity.

  4. The product description of Product P3143, Poly(ethyleneimine) solution, on sigmaaldrich.com appears to give two molecular weight values, Mn and Mw. What do those abbreviations stand for, and why are the two values so different?

    Mn is the number average molecular weight. This corresponds to the "true average" or mean; that is, the sum of the molecular weights divided by the number of molecules. Mw is the weight average molecular weight. This corresponds to the median value; that is, there are as many molecules smaller than the weight average molecular weight as there are larger than the weight average molecular weight. The fact that the two molecular weight values are so different means that there is a large, non-symmetrical distribution of molecular weights; the largest molecules probably have a molecular weight of several million.

  5. What is the purity of Product P3143, Poly(ethyleneimine) solution?

    This product is a polymer with a large molecular weight distribution. It is not amenable to being analyzed by typical chromatographic assays such as HPLC or TLC. Therefore, we do not report an overall purity value for this product.

  6. Do you have any references that describe using Product P3143, Poly(ethyleneimine) solution, in enzyme isolation or for transfections?

    For enzyme isolation, see:J.J. Jendrisak and R.R. Burgess, Biochem., 14(21), 4639-4645 (1975), "A new method for the large-scale purification of wheat germ DNA-dependent RNA polymerase II".This paper describes preparing a 10% stock solution, which was adjusted to pH 7.9 by adding hydrochloric acid.PEI has also been used for the precipitation of nucleic acids in the purification process of aminoacyl-tRNA synthetases:von der Haar, F., "Purification of Aminoacyl-tRNA Synthetases", Methods in Enzymology, 59, 257-267 (1979).For transfections, see the following references:Boussif, O. et al., "A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine." Proc. Natl. Acad. Sci. USA, 92(16), 7297-7301 (1995).Thomas, M. and Klibanov, A.M., "Conjugation to gold nanoparticles enhances polyethylenimine's transfer of plasmid DNA into mammalian cells." Proc. Natl. Acad. Sci. USA, 100(16), 9138-9143 (2003).

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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